Antibiotic Resistance Patterns of E. coli and Staphylococcus aureus Isolated from Hospital wastewater samples of Mysore, Karnataka, South India

Divya S; Jessen George; Divya L; Midhun G; Nazroza Ali; S Suriyanarayanan

Divya S Department of Water and Health, Faculty of Life Sciences, JSS University, SS Nagar, Mysore, Karnataka, India-570015
Jessen George Department of Water and Health, Faculty of Life Sciences, JSS University, SS Nagar, Mysore, Karnataka, India-570015
Divya L Department of Water and Health, Faculty of Life Sciences, JSS University, SS Nagar, Mysore, Karnataka, India-570015
Midhun G Department of Water and Health, Faculty of Life Sciences, JSS University, SS Nagar, Mysore, Karnataka, India-570015
Nazroza Ali Department of Water and Health, Faculty of Life Sciences, JSS University, SS Nagar, Mysore, Karnataka, India-570015
S Suriyanarayanan Department of Water and Health, Faculty of Life Sciences, JSS University, SS Nagar, Mysore, Karnataka, India-570015

Abstract

This study has been carried out to understand the antibiotic resistance patternsof commonly inhabits bacteria like Staphylococcus aureusand gramnegative bacteria E. coli were analyzed from hospital wastewater effluents.Twelve commonly used Pharmaceutical Compounds (PCs) were selected for this study. The PCâ€™s selected for this study were Methicillin (MET), Cephalothin (CEP), Cefazolin (CZ), Cotrimoxazole (COT), Clindamycin (CD), Fusidic acid (FC), Oxicillin (OX), Linezolid (LZ), Faropenem (FAR), Doxycycline (DO), Vancomycin (VA) and Rifamycin (RIF). The resistance, susceptible and intermediate patterns by the gram-positive and gram-negative bacteria towards the selected PCâ€™s were observed. Among the tested antibiotics the gram-positive bacteria, Staphylococcus aureus showed 100% resistance towards the antibiotics of Methicillin (MET), Cephalothin (CEP), Cefazolin (CZ), Cotrimoxazole (COT), Clindamycin (CD), Fusidicacid (FC) and Rifamycin (RIF). The study result shows30% resistance towards Faropenem (FAR)antibiotic. The gramnegative bacteria E. coli even showed 100% resistance towards Methicillin (MET), Cephalothin (CEP), Cefazolin (CZ), Cotrimoxazole (COT), Clindamycin (CD), Fusidic acid (FC) and Rifamycin (RIF). In general the selected PCâ€™s it showed 70% resistance. The source water to the hospital effluent is contaminated with multiple drug resistant coliform bacteria and detection of Staphylococcus spp.