Evaluation of partial characterization of cellulase production by Aspergillus fumigatus SK1 under untreated Oil Palm empty Fruit Bunch (OPEFB) in solid state fermentation

Abstract

Evaluation of utilization of untreated Oil PalmEmpty Fruit Bunch (OPEFB) as a substrate for the reducing sugars by Aspergillus fumigatus SK1 in solid state fermentation (SSF). A pH value of 5 and temperature of 60°Cwere found to be optimal for maximum cellulase enzyme production. Aspergillus fumigatus capable to produce CMCase and ¯-glucosidase enzymes with highest activity indicated as 1.352 and 0.272 U/mL respectively on a sixth day of incubation except FPase which indicated that Aspergillus fumigatus not suitable for FPase production. The partial purification of the cellulase enzyme produced by Aspergillus fumigatus SK1 under untreated Oil Palm empty Fruit Bunch (OPEFB) in solid state fermentation. The endoglucanase (CMCase) proteins were detected as 72.6 kDa and 42.4 kDa bands andmnexoglucanase (FPase) as 72.6 kDa band. The molecular mass of ¯-glucosidase was found to be about 42.4 kDa by SDS-PAGE.