Biochemical studies on the cleavage stage of a fresh water snail (Lymnaea stagnalis) after treatment with colchicine and paclitaxel

Abstract

Lymnaeastagnalisis an herbivores, hermaphroditeand oviparous snail. It is not only pest of aquatic vegetationbut also act as an intermediate host of somepathogenic trematodes. In the present investigation alkaloids were tested for lethal toxicity against Lymnaeastagnalis. The values of LC100, LC50, LC0 and sublethalconcentration were detected out and data was summarizedin Table. 1 and 2. Result indicated that paclitaxelhad a higher toxicity on cleavage stage of Lymnaeastagnalisthan colchicine. In the present investigation cleavageof Lymnaeastagnaliswas treated with different concentrationof alkaloids showed dose and duration dependentgradual changes in the number of protein fractionin the cleavage stage resulted into anomalies in themorphogenesis. Detection of negatively charged proteinfractions in cleavage stage of control and treatedsnails by SDS-PAGE of Lymnaeastagnaliswas assessed in the present investigation. The values were low intreated groups as a consequence of the poor developmentof cleavage due to intoxication of alkaloids.